|
KMID : 0380620130450060639
|
|
Korean Journal of Food Science and Technology
2013 Volume.45 No. 6 p.639 ~ p.699
|
|
|
Development of a Method for Analyzing the Nicotine Content in Synthetic Flavoring Substances as Unauthorized E-cigarette Liquid by Using HPLC
|
|
Kim Jae-Young
Lee Sang-Mok
Chang Moon-Ik
Cho Yoon-Jae
Lee Han-Jin
Chae Young-Sik
Rhee Gyu-Sik
|
|
|
Abstract
|
|
|
|
A simple, sensitive, and specific method for quantifying the nicotine content of synthetic favoring substances (SFS) was developed using high performance liquid chromatography (HPLC) with a photo-diode array detector (PDA). Nicotine was extracted from SFS samples by using an acid-base liquid-liquid extraction method with dichloromethane and distilled water. The nicotine content was quantified by HPLC/PDA (261.9 nm) with a C18 column under a gradient of 10% acetonitrile with 20 mM ammonium formate (ammonia solution adjusted to pH 8.7) to 100% acetonitrile. The calibration curve, analyzed from concentration standards between 0.1 to 2 mg/L, presented linearity with a correlation coefficient (r2)>0.9999. The limit of quantitation (LOQ) of nicotine in SFS was 0.4 mg/kg, and the average recoveries ranged from 76.4% to 96.3%. The repeatability of measurements, expressed as the coefficient of variation (CV%), ranged from 1.74 to 5.12%. This newly developed method for nicotine quantification in SFS can be considered an analytical method with an acceptable level of sensitivity and repeatability.
|
|
|
KEYWORD
|
|
|
nicotine, synthetic flavoring substances, HPLC/PDA, analytical method
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|